Whole genome sequence of Clostridium perfringens: isolated from fecal... - SRA

SRX26781664: Whole genome sequence of Clostridium perfringens: isolated from fecal sample of healthy birds
1 ILLUMINA (Illumina MiSeq) run: 599,748 spots, 235.8M bases, 117.4Mb downloads

Design: Whole genome sequencing libraries were prepared using Nextera XT DNA Library Prep kit (Illumina, Doc# 15031942). Briefly, 12 ng of genomic DNA was fragmented using Nextera transposome and tagged with adapter sequences. The adapter-tagged DNA was amplified using a limited-cycle PCR program to add index adapters and sequences required for sequence cluster generation. The amplified libraries were purified using Sample Purification Beads. The purified libraries were then normalized and combined in equal molar ratios based on their DNA concentrations; the pooled fragments were used for sequencing. The pooled libraries were denatured with NaOH and diluted with hybridization buffer before sequencing.

Submitted by: University of Montreal

Study: Whole genome sequencing of Clostridium perfringens from healthy and necrotic enteritis affected broiler chicken farms

PRJNA1188553 • SRP546473 • All experiments • All runs

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We conducted whole genome sequencing of 27 Clostridium perfringens isolates recovered from both healthy and necrotic enteritis afflicted broiler chickens in Quebec province, Canada, to expand our knowledge about the genetic diversity and virulence traits of this pathogen.

Sample: Isolated from healthy bird

SAMN44841637 • SRS23268265 • All experiments • All runs

Organism: Clostridium perfringens

Library:

Name: MLG_3111

Instrument: Illumina MiSeq

Strategy: WGS

Source: GENOMIC

Selection: RANDOM

Layout: PAIRED

Runs: 1 run, 599,748 spots, 235.8M bases, 117.4Mb

Run	# of Spots	# of Bases	Size	Published
SRR31412630	599,748	235.8M	117.4Mb	2024-11-20

ID:: 36189780

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